t4 dna ligase (New England Biolabs)
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New England Biolabs
t4 dna ligase
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dntps/Deoxynucleotide+(dNTP)+Solution+Set/pm42881293-198-80-87
Average 97 stars, based on 898 article reviews
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 898 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dntps/Deoxynucleotide+(dNTP)+Solution+Set/pm42881293-198-80-87
Average 97 stars, based on 898 article reviews
t4 dna ligase - by Bioz Stars,
2026-10
97/100 stars
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Generated:Article Title: Specific elimination of m.8993T>G mitochondrial haplotype in NARP cybrid cells by CRISPR-Cas9 system. Article Snippet: .. Amplicons were generated in a reaction containing 1× Q5 Reaction Buffer (NEB), 12.5 pmol of each primer, 0.2 mM of Polymerase Chain Reaction:Article Title: RNase P/MRP subunits chaperone telomerase holoenzyme assembly in fission yeast. Article Snippet: MaP buffer (8 μl) was added, and samples were incubated at 42 °C for 2 min. Superscript II reverse transcriptase (1 μl; ThermoFisher, 18064014) was added, and reactions were incubated at 42 °C for 3 h, followed by inactivation at 70 °C for 15 min. cDNA was purified using G-25 microspin columns (Cytiva, 27532501). .. PCR was carried out in 50 μl reactions with 1× Q5 Reaction Buffer, 200 μM Article Title: Pooled CRISPRi screening reveals fungal-specific drug target candidates. Article Snippet: The rising rate of drug-resistant fungal infections and the emergence of intrinsically resistant pathogens pose growing clinical challenges.. Because fungi are closely related to mammals, developing antifungals without toxic off-target effects is difficult.. Targeted gene repression can model drug-mediated inhibition and reveal gene dosage sensitivity, but traditional approaches in the fungal pathogen Candida albicans are labour intensive and low throughput. Article Title: Precision Fermentation of Recombinant Myofibrillar Proteins for Future Foods Article Snippet: Primers were designed and PCR conditions determined using NEBuilder. .. PCR reactions were carried out using NEB Q5 High-Fidelity DNA Polymerase (NEB #M0491) and Lysis:Article Title: SCIMETAR-seq tracks immunophenotype, demethylation, mutations, and transcriptomes in single cells undergoing HMA therapy Article Snippet: .. Single cells were sorted into 96-well plates containing 4 μL of lysis buffer per well (Tween-20 (0.1%; Sigma-Aldrich, P9416-50ML), recombinant RNase inhibitor (0.53 U/μL; New England Biolabs, M0314L), PEG 8000 (6.25%; Sigma-Aldrich, P1458-25ML), EDTA (0.1 mM; Sigma-Aldrich, 03690-100ML), Tris-HCl (2.5 mM; pH ∼7.0, Thermo Fisher Scientific, AM9850G), oligo(dT) primer (0.625 μM; Supplementary Table 3), and Recombinant:Article Title: SCIMETAR-seq tracks immunophenotype, demethylation, mutations, and transcriptomes in single cells undergoing HMA therapy Article Snippet: .. Single cells were sorted into 96-well plates containing 4 μL of lysis buffer per well (Tween-20 (0.1%; Sigma-Aldrich, P9416-50ML), recombinant RNase inhibitor (0.53 U/μL; New England Biolabs, M0314L), PEG 8000 (6.25%; Sigma-Aldrich, P1458-25ML), EDTA (0.1 mM; Sigma-Aldrich, 03690-100ML), Tris-HCl (2.5 mM; pH ∼7.0, Thermo Fisher Scientific, AM9850G), oligo(dT) primer (0.625 μM; Supplementary Table 3), and Reverse Transcription:Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, Article Title: sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cells Article Snippet: Around 100–150 μl beads were centrifuged in a 1.5-ml Eppendorf tube at 1500 rcf for 2 min to obtain packed beads. .. After aspirating residual buffer from the pelleted beads, the tube was loaded onto the corresponding inlet in the microfluidics setup. (iii) Reverse transcription/lysis mix inlet: 150 μl RT/lysis mix consisted of 30 μl 10× StellarScript buffer, 15μl StellarScript RT enzyme 200 U/μl (StellarScript HT Reverse Transcriptase Kit, Watchmaker, cat #WM-7K0070), 10 μl murine RNase inhibitor (NEB, cat #M0314), 15 μl 1 M Tris–HCl (pH 8.0), 10 μl 0.1 M Dithiothreitol (DTT), 9 μl 10% (v/v) IGEPAL CA-630 (Sigma–Aldrich, cat #I8896), 6 μl 25 mM Incubation:Article Title: Systematic assessment of diverse RNA modifications using nanopore direct RNA sequencing Article Snippet: .. Reverse transcription was performed by adding a master mix of nuclease-free water, |
